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JoVE Journal Genetics
Tvåriktad retroviral integrationsplats PCR-metodik och kvantitativ dataanalys arbetsflöde
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Tvåriktad retroviral integrationsplats PCR-metodik och kvantitativ dataanalys arbetsflöde

Article DOI: 10.3791/55812-v • 12:53 min • June 14th, 2017
June 14th, 2017 •
*1,2, *3, 1, 3, 4, 1,5, 6
1UCLA AIDS Institute, University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics, University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics, University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups, Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine, University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University (OSU)
* These authors contributed equally

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Detta manuskript beskriver experimentell procedur och mjukvaruanalys för en dubbelriktad integreringsställeanalys som samtidigt kan analysera uppströms och nedströms vektor-värd-kryssnings-DNA. Tvåriktiga PCR-produkter kan användas för någon nedströms sekvenseringsplattform. Den resulterande data är användbar för en hög genomströmning, kvantitativ jämförelse av integrerade DNA-mål.

Tags

Genetik Utgåva 124 Retrovirus Integrationsställen Nästa generationens sekvensering Retroviral genterapi Stamceller Cancers Cellbar streckkodning Bioinformatik
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