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Monitoring ER / SR Calciumvrijstelling met de gerichte Ca
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Monitoring ER / SR Calciumvrijstelling met de gerichte Ca

Article DOI: 10.3791/55822-v • 12:30 min • May 19th, 2017
May 19th, 2017 •
1, 1, 1, 1
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

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Wij presenteren protocollen voor de toepassing van onze genetisch gecodeerde calciumindicator (GECI) CatchER + voor het monitoren van snelle calcium transiënten in het endoplasmatische / sarcoplasmatische reticulum (ER / SR) van HEK293 en skeletspier C2C12 cellen met behulp van real-time fluorescentiemicroscopie. Een protocol voor de in-situ K d meting en kalibratie wordt ook besproken.

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Biochemie Uitgave 123 Calciumbeeldvorming Sarko-Endoplasmatisch Reticulum Genetisch Gecodeerde Calciumindicator Calciumsignaal CatchER Fluorescentiemicroscopie
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