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JoVE Journal Developmental Biology
Ljusmedierad reversibel modulering av den mitogenaktiverade proteinkinasvägen under celldifferentiering och
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Ljusmedierad reversibel modulering av den mitogenaktiverade proteinkinasvägen under celldifferentiering och

Article DOI: 10.3791/55823-v • 09:32 min • June 15th, 2017
June 15th, 2017 •
*1, *2, 1, 1, 1, 2, 2, 1,3,4
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 3Neuroscience Program, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Detta protokoll beskriver en optogenetisk strategi för att modulera mitogenaktiverad proteinkinas (MAPK) aktivitet under celldifferentiering och Xenopus embryonal utveckling. Denna metod möjliggör reversibel aktivering av MAPK-signalvägen i däggdjurscellkulturen och i multicellulära levande organismer, som Xenopus- embryon, med hög rumlig och tidsmässig upplösning.

Tags

Developmental Biology Optogenetics protein-protein interaktioner PC12 cell differentiering, Embryonal utveckling kryptokrom CRY2-CIBN bicistronic Raf / MEK / ERK-kinas signaleringskaskad
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