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JoVE Journal
Developmental Biology

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在细胞分化过程中光介导的可逆调节丝裂原活化蛋白激酶途径
 
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在细胞分化过程中光介导的可逆调节丝裂原活化蛋白激酶途径

Article DOI: 10.3791/55823-v 09:32 min June 15th, 2017
June 15th, 2017

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Summary

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Click here for the English version.

该协议描述了在细胞分化和非洲爪蟾胚胎发育过程中调节丝裂原活化蛋白激酶(MAPK)活性的光遗传策略。这种方法允许哺乳动物细胞培养物和多细胞活生物体如非洲爪蟾胚胎中具有高空间和时间分辨率的可逆激活MAPK信号通路。

Tags

发育生物学,第124期,光致遗传学,蛋白质 - 蛋白质相互作用,PC12细胞分化,
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