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September 15, 2017
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The overall goal of this screening assay is to characterize novel endothelial regulators involved in the inflammatory response of interest for designing new therapeutic strategies against inflammatory disorders.This method can help answer key questions in the pathology field such as defining novel endothelial-targeting drugs able to attenuate inflammatory-related diseases.The main advantage of this endothelial protocol is that describes assay technology that establishes the basis for exploring novel mechanisms involved in the regulation of the inflammatory response.Demonstrating this methodology will be Maria Angeles Higueras, a post-doc from our lab and Sandra Herranz, a technician from our lab.To begin, culture MLEC-04 cells in complete DMEM F12 medium supplemented with 10%FBS and Pen-Strep.Incubate the cells in a biological incubator at 37
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Endothélium vasculaire contrôle étroitement le recrutement des leucocytes. Extravasation de leucocyte inadéquate contribue aux maladies inflammatoires humaines. La recherche de nouveaux éléments régulateurs d’activation endothéliale est donc nécessaire de concevoir des traitements améliorés pour troubles inflammatoires. Nous décrivons ici une méthodologie globale afin de caractériser les nouveaux régulateurs endothéliales qui peuvent de modifier leucocytes traite au cours de l’inflammation.
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Higueras, M. Á., Jiménez-García, L., Herranz, S., Hortelano, S., Luque, A. Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response. J. Vis. Exp. (127), e55824, doi:10.3791/55824 (2017).
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