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In Vitro Polimerização de F-Actina no início endossomos
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In Vitro Polimerização de F-Actina no início endossomos

Article DOI: 10.3791/55829-v • 12:15 min • August 28th, 2017
August 28th, 2017 •
1,2, 1, 1, 1, 1
1Department of Biochemistry, University of Geneva, 2Department of Fundamental Microbiology, University of Lausanne

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Funções de endossomo precoce dependem de polimerização de F-Actina. Aqui, descrevemos um ensaio baseado em microscopia em vitro que reconstitui a nucleação e polimerização de F-Actina nas membranas de CDDP precoce em tubos de ensaio, tornando assim esta complexa série de reações passíveis de bioquímicos e genéticos manipulações.

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Bioquímica edição 126 actina endossomo RAB5 citoesqueleto tráfego ensaio em vitro
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