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JoVE Journal Biochemistry
In Vitro Polymerisatie van F-actine op vroege Endosomen
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In Vitro Polymerisatie van F-actine op vroege Endosomen

Article DOI: 10.3791/55829-v • 12:15 min • August 28th, 2017
August 28th, 2017 •
1,2, 1, 1, 1, 1
1Department of Biochemistry, University of Geneva, 2Department of Fundamental Microbiology, University of Lausanne

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Vroege endosome functies is afhankelijk van F-actine polymerisatie. Hier beschrijven we een microscopie gebaseerde in vitro -assay die reconstitutes de nucleatie en polymerisatie van de F-actine op vroege endosomal membranen in proefbuizen, waardoor deze complexe reeks reacties vatbaar voor biochemische en genetische manipulaties.

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Biochemie kwestie 126 actine endosome RAB5 cytoskelet verkeer in vitro assay
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