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JoVE Journal Genetics
In Vitro Bioluminescentie Assay te karakteriseren Circadian Rhythm in borstklier epitheliale cellen
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In Vitro Bioluminescentie Assay te karakteriseren Circadian Rhythm in borstklier epitheliale cellen

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Een in vitro bioluminescentie test om te bepalen van cellulaire circadiane ritme in de borstklier epitheliale cellen wordt gepresenteerd. Deze methode maakt gebruik van zoogdiercellen verslaggever plasmiden gedestabiliseerd luciferase onder de controle van de periode 2 gen promotor uitdrukken. Het kan worden aangepast aan andere celtypes te evalueren orgel-specifieke effecten op de circadiane ritme.

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Genetica kwestie 127 circadiane ritme periode 2 promotor activiteit, in vitro real-time bioluminescentie assay luciferase expressie vector destabiliseren firefly luciferase plasmide transfectie.
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