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Journal / Genetics / In-vitro- Biolumineszenz-Assay zirkadianen…
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JoVE Journal Genetics
In-vitro- Biolumineszenz-Assay zirkadianen Rhythmus in Mamma Epithelzellen zu charakterisieren
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In-vitro- Biolumineszenz-Assay zirkadianen Rhythmus in Mamma Epithelzellen zu charakterisieren

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Ein in-vitro- Biolumineszenz-Assay zellulären zirkadianen Rhythmus in Mamma Epithelzellen bestimmen wird vorgestellt. Diese Methode nutzt Säugerzelle Reporter Plasmide destabilisierte Luciferase unter der Kontrolle des Veranstalters Periode 2 gen zum Ausdruck zu bringen. Es kann zu anderen Zelltypen auszuwertende Organ-spezifischen Auswirkungen auf die zirkadianen Rhythmus angepasst werden.

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Genetik Ausgabe 127 zirkadianen Rhythmus Periode 2 Promotoraktivität, in-vitro- Echtzeit-Biolumineszenz-Assay Luciferase Expressionsvektor destabilisiert Firefly Luciferase Plasmid Transfektion.
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