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Genetics

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JoVE Journal Genetics
In Vitro Bioluminescens Assay at karakterisere døgnrytme i Brystkirtlerne epitelceller
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In Vitro Bioluminescens Assay at karakterisere døgnrytme i Brystkirtlerne epitelceller

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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En in vitro- bioluminescens analyse til at bestemme cellulære døgnrytme i Brystkirtlerne epitelceller er præsenteret. Denne metode udnytter pattedyr celle reporter plasmider udtrykker destabiliseret luciferase under kontrol af periode 2 gen promotor. Det kan tilpasses til andre celletyper til at evaluere orgel-specifikke virkninger på døgnrytme.

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Genetik sag 127 Circadian rhythm periode 2 promoter aktivitet, in vitro- real-time bioluminescens assay luciferase udtryk vektor destabiliseret firefly luciferase plasmid Transfektion.
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