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In Vitro Bioluminescens analysen som karakteriserer døgnrytmen i Mammary epitelceller
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In Vitro Bioluminescens analysen som karakteriserer døgnrytmen i Mammary epitelceller

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Bioluminescens i vitro analysen å bestemme mobilnettet døgnrytmen i mammary epitelceller presenteres. Denne metoden bruker pattedyr celle reporter plasmider uttrykke ustabil luciferase under kontroll av periode 2 gen arrangøren. Det kan tilpasses andre celletyper evaluere organ-spesifikke effekter på døgnrytmen.

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Genetikk problemet 127 Circadian rytme periode 2 formidler aktivitet, i vitro sanntid bioluminescens analysen luciferase uttrykk vektor ustabil firefly luciferase plasmider transfection.
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