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Journal / Genetics / In Vitro Ensayo de bioluminiscencia para la…
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Genetics

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JoVE Journal Genetics
In Vitro Ensayo de bioluminiscencia para la caracterización del ritmo circadiano en células epiteliales mamarias
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In Vitro Ensayo de bioluminiscencia para la caracterización del ritmo circadiano en células epiteliales mamarias

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Se presenta un ensayo de bioluminiscencia en vitro para determinar del ritmo circadiano celular en células epiteliales mamarias. Este método utiliza plásmidos de reportero de células de mamífero expresando desestabilizada luciferasa bajo el control del promotor del gen de periodo 2 . Puede ser adaptado a otros tipos de células para evaluar efectos de órgano-específicas en el ritmo circadiano.

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Genética número 127 ritmo circadiano periodo 2 promotor actividad en vitro ensayo de bioluminiscencia en tiempo real vector de la expresión de luciferasa desestabilizó la luciferasa de la luciérnaga transfección del plásmido.
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