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Journal / Genetics / Vitro Sirkadiyen ritim meme epitel hücreleri…
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JoVE Journal Genetics
Vitro Sirkadiyen ritim meme epitel hücreleri ayırdetmek için bioluminescence tahlil
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Vitro Sirkadiyen ritim meme epitel hücreleri ayırdetmek için bioluminescence tahlil

Article DOI: 10.3791/55832-v • 11:56 min • September 28th, 2017
September 28th, 2017 •
1, 2, 2, 1, 1
1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

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Hücresel sirkadiyen ritim meme epitel hücreleri belirlemek için bir vitro bioluminescence tahlil sunulur. Bu yöntem memeli hücre muhabir plazmid istikrarsızlığın luciferase dönem 2 gen düzenleyici denetimi altında ifade kullanır. Sirkadiyen ritim organ özgü etkileri değerlendirmek için diğer hücre tipleri için uyarlanabilir.

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Genetik sayı: 127 sirkadiyen ritim dönem 2 organizatörü, vitro gerçek zamanlı bioluminescence tahlil etkinlik luciferase ifade vektör ateş böceği luciferase plazmid transfection dengeleri bozdu.
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