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Journal / Medicine / İzole edilen fare kalpten interstisyel transüdao…
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İzole edilen fare kalpten interstisyel transüdao toplanması için ters kalp modeli
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İzole edilen fare kalpten interstisyel transüdao toplanması için ters kalp modeli

Article DOI: 10.3791/55849-v • 07:59 min • June 20th, 2017
June 20th, 2017 •
*1,2, *1,2, 2, 3, 3, 1, 2
1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University
* These authors contributed equally

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Bu protokol, izole edilmiş perfüze edilmiş fare kalpten kardiyak interstisyel sıvıyı toplamak için bir yöntemi açıklamaktadır. İnterstisyel transüdaatı koroner venöz atık perfüzattan fiziksel olarak ayırmak için, Langendorff perfüze edilen kalp ters çevrilir ve kalp yüzeyinde oluşan transüda (interstisyel sıvı) yumuşak bir lateks kapak kullanılarak toplanır.

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Tıp Sayı 124 Langendorff perfüzyon ters kalp sıçan interstisyel sıvı miyokard infarktüsü HPLC proteomik
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