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JoVE Journal Neuroscience
Spinal Cord Neurons Isolatie en Cultuur van Neonatale Muizen
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Spinal Cord Neurons Isolatie en Cultuur van Neonatale Muizen

Article DOI: 10.3791/55856-v • 07:49 min • July 11th, 2017
July 11th, 2017 •
1, 1, 1, 1
1Department of Surgery, Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus

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Deze studie presenteert een techniek voor de isolatie van neuronen van WT-neonatale muizen. Het vereist de zorgvuldige dissectie van het ruggenmerg van de neonatale muis, gevolgd door de scheiding van neuronen uit het ruggenmergweefsel door mechanische en enzymatische splitsing.

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Neurowetenschappen Uitgave 125 Ruggenmerg Muizenneuron Neonatale Muizen Celcultuur Zuivering Dichtheidsgradiënt Papain NeuN MAP2
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