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JoVE Journal Neuroscience
Isolamento e cultura dei neuroni del midollo spinale da topi neonatali
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Isolamento e cultura dei neuroni del midollo spinale da topi neonatali

Article DOI: 10.3791/55856-v • 07:49 min • July 11th, 2017
July 11th, 2017 •
1, 1, 1, 1
1Department of Surgery, Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus

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Questo studio presenta una tecnica per l'isolamento dei neuroni da topi neonatali WT. Richiede l'attenta disezione del midollo spinale dal topo neonatale, seguita dalla separazione dei neuroni dal tessuto del midollo spinale attraverso la scissione meccanica ed enzimatica.

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Neuroscienza Edizione 125 Cordone spinale neurone del mouse topi neonatali coltura cellulare purificazione gradiente di densità papain NeuN MAP2
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