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Aislamiento y Cultura de Neuronas de la Médula Espinal de Ratones Neonatales
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Aislamiento y Cultura de Neuronas de la Médula Espinal de Ratones Neonatales

Article DOI: 10.3791/55856-v • 07:49 min • July 11th, 2017
July 11th, 2017 •
1, 1, 1, 1
1Department of Surgery, Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus

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Este estudio presenta una técnica para el aislamiento de neuronas de ratones neonatales WT. Requiere la cuidadosa disección de la médula espinal del ratón neonatal, seguida de la separación de las neuronas del tejido de la médula espinal mediante escisión mecánica y enzimática.

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Neurociencia Número 125 Médula espinal neurona de ratón ratones neonatales cultivo celular purificación gradiente de densidad papaína NeuN MAP2
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