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JoVE Journal Neuroscience
Modeling Neuronal død og Degeneration i musen primære cerebellare granulet neuroner
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Modeling Neuronal død og Degeneration i musen primære cerebellare granulet neuroner

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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Denne protokol beskriver en simpel metode til isolering og dyrkning af primære mus cerebral granulet neuroner (CGNs) fra 6-7 dage gamle hvalpe, effektiv transduktion af CGNs for tab og gevinst på funktion undersøgelser, og modellering NMDA-induceret neuronal excitotoxicity, lav-kalium-induceret celledød, DNA-skader og oxidativt stress ved hjælp af den samme kultur model.

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Neurovidenskab sag 129 lillehjernen cerebellare granulet neuroner (CGN) Adenovirus Lentiviral transduktion Excitotoxicity N-methyl-D-aspartat (NMDA) apoptose kaliumchlorid (KCl) Camptothecin hydrogenperoxid (H2O2)
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