Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Gelijktijdige meting van HDAC1 en HDAC6…
JoVE Journal
Chemistry

A subscription to JoVE is required to view this content.

JoVE Journal Chemistry
Gelijktijdige meting van HDAC1 en HDAC6 activiteit in HeLa cellen met behulp van UHPLC-MS
Bulk Cyclic Voltammetry Measurements of Nile Blue (Video) | JoVE
Journal (Chemistry)
Bulk Cyclic Voltammetry Measurements of Nile Blue (Video) | JoVE
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
Journal (Chemistry)
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
 
Click here for the English version

Gelijktijdige meting van HDAC1 en HDAC6 activiteit in HeLa cellen met behulp van UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

De huidige methode dient ter identificatie van isovorm-specifieke remmers van Histon deacetylases (HDAC) in HeLa cellen door de UHPLC-MS-analyse van meerdere substraten. Dit is een antilichaam-vrije methode ontwikkeld om te reflecteren van HDAC1 en HDAC6 activiteit in de levende cel omgeving, in tegenstelling tot één-isovorm cel-gratis testen.

Tags

Chemie kwestie 126 HDAC1 HDAC6 remmers cel-gebaseerde assay UHPLC-MS HeLa cellen
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter