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Samtidig måling af HDAC1 og HDAC6 aktivitet i HeLa celler ved hjælp af UHPLC-MS
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Samtidig måling af HDAC1 og HDAC6 aktivitet i HeLa celler ved hjælp af UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

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Den nuværende metode tjener til at identificere isoform-specifikke hæmmere af Histon deacetyltransferase (HDAC) i HeLa celler af UHPLC-MS analysen af flere substrater. Dette er et antistof-fri metode udviklet for at afspejle HDAC1 og HDAC6 aktivitet i levende celle miljø, i modsætning til single-isoform celle-fri assays.

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Kemi sag 126 HDAC1 HDAC6 hæmmere celle-baserede assay UHPLC-MS HeLa celler
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