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Journal / Biology / ODELAY: En storskala metode til kvantificering af …
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ODELAY: En storskala metode til kvantificering af gærvækst i flere parametre

Article DOI: 10.3791/55879-v • 11:19 min • July 3rd, 2017
July 3rd, 2017 •
1, 1,2, 1, 1,2
1Institute for Systems Biology, 2Center for Infectious Disease Research

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Vi præsenterer en metode til kvantificering af vækstfænotyper af individuelle gærceller, da de vokser ind i kolonier på faste medier ved anvendelse af tidsforskydningsmikroskopi betegnet, En-celledobbeltbedømmelse af levende arrays af gær (ODELAY). Befolknings heterogenitet af genetisk identiske celler, der vokser ind i kolonier, kan observeres og kvantificeres direkte.

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Cellular Biology Væksthastighed forsinketid bæreevne fitness vurdering gær fænotype analyse
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