Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 选择依赖和独立生成CRISPR /…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Journal (Genetics)
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Journal (Genetics)
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
 
Click here for the English version

选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除

Article DOI: 10.3791/55903-v • 11:35 min • June 16th, 2017
June 16th, 2017 •
1, 1, 1
1Cell Biology and Neuroscience, University of California, Riverside

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

遗传操纵体细胞系的能力的最新进展对于基础和应用研究具有巨大的潜力。在这里，我们提出了CRISPR / Cas9在哺乳动物细胞系中产生敲除生产和筛选的两种方法，使用和不使用选择标记。

Tags

遗传学，第124期，CRISPR / Cas9，同源驱动修复，非同源末端连接，斑点印迹，克隆选择，筛选
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter