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I situ Immunofluorescerande färgning av autophagy i muskelstamceller
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I situ Immunofluorescerande färgning av autophagy i muskelstamceller

Article DOI: 10.3791/55908-v • 08:35 min • June 12th, 2017
June 12th, 2017 •
1,2, 3, 4, 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

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Aktiv autofagi är förknippad med produktiv muskelregenerering, vilket är väsentligt för aktivering av muskelstamceller (MuSC). Här tillhandahåller vi ett protokoll för in situ- detektering av LC3, en autofagmarkör i MyoD-positiva MuSCs av muskelvävnadssektioner från kontroll och skadade möss.

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Cellbiologi Utgåva 124 Autophagy muskelstamceller (satelliter) muskelregenerering LC3 MYOD muskelvävnadssektioner
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