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In situ Immunofluorescerende farvning af autophagy i muskelstamceller
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In situ Immunofluorescerende farvning af autophagy i muskelstamceller

Article DOI: 10.3791/55908-v • 08:35 min • June 12th, 2017
June 12th, 2017 •
1,2, 3, 4, 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

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Aktiv autofagi er forbundet med produktiv muskelregenerering, hvilket er afgørende for aktivering af muskelstamceller (MuSC). Her tilvejebringer vi en protokol til in situ detektion af LC3, en autophagy markør i MyoD-positive MuSCs af muskelvævssektioner fra kontrol og skadede mus.

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Cellular Biology Autophagy muskel stamceller (satellitter) muskelregeneration LC3 MYOD muskelvævssektioner
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