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Journal / Biology / Tinción Inmunofluorescente In Situ de Autofagia…
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Tinción Inmunofluorescente In Situ de Autofagia en Células Madre Musculares
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Tinción Inmunofluorescente In Situ de Autofagia en Células Madre Musculares

Article DOI: 10.3791/55908-v • 08:35 min • June 12th, 2017
June 12th, 2017 •
1,2, 3, 4, 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

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La autofagia activa se asocia con la regeneración muscular productiva, que es esencial para la activación de las células madre musculares (MuSC). Aquí, proporcionamos un protocolo para la detección in situ de LC3, un marcador de autofagia en MyoD-positivos MuSCs de secciones de tejido muscular de control y los ratones lesionados.

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Biología Celular Número 124 Autofagia células madre musculares (satélites) regeneración muscular LC3 MYOD secciones de tejido muscular
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