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Journal / Bioengineering / 使用CRISPR并发剂的小鼠小肠组织中多基因敲除的方案…
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Bioengineering

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JoVE Journal Bioengineering
使用CRISPR并发剂的小鼠小肠组织中多基因敲除的方案
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使用CRISPR并发剂的小鼠小肠组织中多基因敲除的方案

Article DOI: 10.3791/55916-v • 11:53 min • July 12th, 2017
July 12th, 2017 •
1,2, 1,2, 1, 1, 3, 1,2
1Wellcome Trust - Medical Research Council Stem Cell Institute, University of Cambridge, 2Department of Genetics, University of Cambridge, 3Department of Pathology, Yonsei University College of Medicine

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该协议描述了将多个单个引导RNA克隆到一个引导RNA并行载体中的步骤，其特别用于使用CRISPR / Cas9技术创建多基因敲除。肠组织中双重敲除的产生显示为该方法的可能应用。

Tags

生物工程，第125期，CRISPR / Cas9，敲除，有机体，肠，电穿孔，旁系同源物，Wnt。
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