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Journal / Developmental Biology / Isolierung von menschlichen Myoblasten,…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolierung von menschlichen Myoblasten, Beurteilung der myogenen Differenzierung und speicherbetriebenen Kalziumeintrittsmessung
Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
Journal (Developmental Biology)
Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
Journal (Developmental Biology)
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
 
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Isolierung von menschlichen Myoblasten, Beurteilung der myogenen Differenzierung und speicherbetriebenen Kalziumeintrittsmessung

Article DOI: 10.3791/55918-v • 10:45 min • July 26th, 2017
July 26th, 2017 •
*1, *2, 2,3, 3
1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center
* These authors contributed equally

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Hier beschreiben wir eine Methodik, um eine reine Population von menschlichen Myoblasten aus erwachsenem Muskelgewebe zu erhalten. Diese Zellen werden verwendet, um die In-vitro- Skelettmuskeldifferenzierung zu untersuchen und insbesondere Proteine ​​zu untersuchen, die an der Ca 2+ -Signalisierung beteiligt sind.

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Entwicklungsbiologie Ausgabe 125 myogene Zellen Myoblastdifferenzierung Ca speicherbetrieb Ca markierungen der differenzierung fluoreszenz-aktivierte zellsortierung
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