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Kvantitative lokalisering af Golgi Protein af Imaging dens centrum af fluorescens masse

Article DOI: 10.3791/55996-v • 13:08 min • August 10th, 2017
August 10th, 2017 •
1, 1, 1, 2,3, 1
1School of Biological Sciences, Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing, National University of Singapore

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Den præcise lokalisering af Golgi beboere er afgørende for forståelsen af Golgi cellulære funktioner. Men konventionelle Optisk mikroskopi er afskåret fra løse sub-Golgi strukturen. Her beskriver vi protokol for en konventionel mikroskopi baseret super-resolution metode til at bestemme kvantitativt sub-Golgi lokalisering af et protein.

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Cellulær biologi sag 126 Golgi Golgi mini stack protein lokalisering kvantitative lokalisering Fluorescens mikroskopi super-resolution billeddannelse.
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