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JoVE Journal Developmental Biology
Omprogrammering primära fostervatten och membran celler att Pluripotency Xeno-fria förhållanden
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Omprogrammering primära fostervatten och membran celler att Pluripotency Xeno-fria förhållanden

Article DOI: 10.3791/56003-v • 09:34 min • November 27th, 2017
November 27th, 2017 •
1, 2, 1, 3, 3,4,5, 1, 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

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Det här protokollet beskriver omprogrammering av primära amniotiska vätskan och membran mesenkymala stamceller till inducerade pluripotenta stamceller med en icke-integrerande episomal metod i helt kemiskt definierade villkor. Förfaranden utvinning, kultur, omprogrammering och karakterisering av de resulterande inducerade pluripotenta stamcellerna av stränga metoder är detaljerade.

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