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Journal / Biochemistry / 질량 분석 기반 식별 RNA 바인딩 영역에 대 한 샘플…
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Biochemistry

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JoVE Journal Biochemistry
질량 분석 기반 식별 RNA 바인딩 영역에 대 한 샘플 준비
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Journal (Biochemistry)
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
Journal (Biochemistry)
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
 
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질량 분석 기반 식별 RNA 바인딩 영역에 대 한 샘플 준비

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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우리는 RNA 의무적인 단백질을 확인 하 고 중재 하는 UV photocrosslinking 및 질량 분석을 사용 하 여 라이브 셀에서 그들의 RNA 바인딩 영역 지도를 프로토콜을 설명 합니다.

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생화학 문제 127 단백질 RNA 상호 작용 RNA 바인딩 도메인 RNA 바인딩 영역 질량 분석 noncoding RNAs 자외선 가교
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