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Journal / Biochemistry / Kütle spektrometresi tabanlı kimlik RNA-bağlama…
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Biochemistry

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JoVE Journal Biochemistry
Kütle spektrometresi tabanlı kimlik RNA-bağlama bölgelerinin için numune hazırlama
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Kütle spektrometresi tabanlı kimlik RNA-bağlama bölgelerinin için numune hazırlama

Article DOI: 10.3791/56004-v • 10:52 min • September 28th, 2017
September 28th, 2017 •
1,4, 1,2, 1,3, 1,3, 1,2
1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

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Biz RNA bağlanıcı proteinler tanımlamak ve kendi RNA bağlayıcı bölgeleri UV-aracılı photocrosslinking ve kütle spektrometresi kullanılarak canlı hücrelerdeki eşlemek için bir protokol tanımlamak.

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Biyokimya sayı: 127 Protein-RNA etkileşimleri RNA-bağlayıcı etki RNA-bağlama bölgesi kütle spektrometresi kodlamayan RNA'ların UV crosslinking
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