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Biochemistry

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JoVE Journal Biochemistry
En filtreringsbaseret metode til fremstilling af højkvalitetsnuclei fra tværbundet skeletsmuskel til chromatinimmunpræcipitation
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En filtreringsbaseret metode til fremstilling af højkvalitetsnuclei fra tværbundet skeletsmuskel til chromatinimmunpræcipitation

Article DOI: 10.3791/56013-v • 10:04 min • July 6th, 2017
July 6th, 2017 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston

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Vi præsenterer en filtreringsbaseret protokol til isolering af kerne af høj kvalitet fra tværbundet museskeletmuskulatur, hvor vi fjernede behovet for ultracentrifugering, hvilket gør det let anvendeligt. Vi viser, at kromatin fremstillet ud fra kernerne er egnet til chromatinimmunpræcipitation og sandsynligvis chromatinimmunpræcipitationssekventeringsundersøgelser.

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Biokemi udgave 125 skeletmuskulatur tværbinding kerneisolation chromatinimmunudfældning sekventiel filtrering cirkadian tid
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