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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Article DOI: 10.3791/56027-v • 10:51 min • July 14th, 2017
July 14th, 2017 •
1, 1, 1
1Department of Dermatology, School of Medicine, UC San Diego

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Summary
Automatic Translation

Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.

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Isolation Culture Primary Mouse Keratinocytes Neonatal Mouse Skin Adult Mouse Skin In-vitro Differentiation Innate Immune Responses UVb Exposure Epidermal Biology Keratinocyte Differentiation External Stimuli Serum Culture Wild Type C57 BL6 Mouse Postnatal Day Zero To Two Limbs Removal Skin Peeling PBS Rinse Dispase Digestion Buffer
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