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JoVE Journal
Biology

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来自新生儿和成年鼠皮肤的原代小鼠角化细胞的分离和培养
 
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来自新生儿和成年鼠皮肤的原代小鼠角化细胞的分离和培养

Article DOI: 10.3791/56027-v 10:51 min July 14th, 2017
July 14th, 2017

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Summary

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Click here for the English version.

表皮角质形成细胞形成功能性皮肤屏障,位于主体防御的前线,防止外部环境污染。在这里,我们描述了从新生儿和成年小鼠皮肤分离和原代培养表皮角化细胞的方法,以及从角质形成细胞诱导终末分化和UVB引发的炎症反应。

Tags

细胞生物学,第125期,皮肤表皮,表皮角化细胞,皮肤屏障,角化细胞分化,UVB照射,TNF释放
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