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Journal / Biochemistry / Semi-automatiske Biopanning af bakteriel Display…
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Biochemistry

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JoVE Journal Biochemistry
Semi-automatiske Biopanning af bakteriel Display biblioteker for peptid affinitet reagens opdagelse og analyse af resulterende isolater
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Semi-automatiske Biopanning af bakteriel Display biblioteker for peptid affinitet reagens opdagelse og analyse af resulterende isolater

Article DOI: 10.3791/56061-v • 13:49 min • December 6th, 2017
December 6th, 2017 •
1, 1, 1
1Sensors and Electron Devices Directorate, US Army Research Laboratory

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Biopanning bakteriel display biblioteker er en gennemprøvet teknik til opdagelsen af peptid affinitet reagenser, en robust alternativ til antistoffer. Den semi-automatiske sorteringsmetoden heri er strømlinet biopanning for at mindske forekomsten af falske positiver. Her viser vi den tankeproces og metoder, der anvendes i evaluering af kandidater og minimere downstream analyse.

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Biokemi spørgsmålet 130 bakteriel display biopanning peptid affinitet reagens autoMACS biosensing
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