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Immunology and Infection

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JoVE Journal Immunology and Infection
Geração de variantes de fuga de neutralizar os anticorpos monoclonais de vírus da gripe
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Geração de variantes de fuga de neutralizar os anticorpos monoclonais de vírus da gripe

Article DOI: 10.3791/56067-v • 07:55 min • August 29th, 2017
August 29th, 2017 •
1,2, 1,2, 1,2, 1,2, 3, 3, 1, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago

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Nós descrevemos um método pelo qual podemos identificar resíduos críticos necessários para a ligação de anticorpos monoclonais humanos ou murino que se destinam a hemaglutinina viral do vírus da influenza A. O protocolo pode ser adaptado para outras glicoproteínas de superfície do vírus e suas correspondentes anticorpos neutralizantes.

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Imunologia edição 126 vírus da gripe anticorpos monoclonais variantes de fuga microbiologia virologia
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