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Journal / Biochemistry / Bestemmelse af glycogenindholdet i cyanobakterier
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Biochemistry

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JoVE Journal Biochemistry
Bestemmelse af glycogenindholdet i cyanobakterier
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Bestemmelse af glycogenindholdet i cyanobakterier

Article DOI: 10.3791/56068-v • 07:04 min • July 17th, 2017
July 17th, 2017 •
1, 2, 3
1Carlsberg Research Laboratory, 2Department of Biology, University of Copenhagen, 3Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen

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Her præsenterer vi et pålideligt og let assay til at måle glycogenindholdet i cyanobakterielle celler. Fremgangsmåden indebærer udfældning, selekterbar depolymerisering og påvisning af glucoserester. Denne metode er velegnet til både vildtype og genetisk manipulerede stammer og kan lette metabolisme af cyanobakterier.

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Biochemistry Cyanobacteria, glycogen glucoseoxidase peroxidase amylase amyloglucosidase
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