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Journal / Genetics / Generation af indfødte kromatin…
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Genetics

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JoVE Journal Genetics
Generation af indfødte kromatin Immunoprecipitation sekventering biblioteker for Nucleosome tæthed analyse
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Generation af indfødte kromatin Immunoprecipitation sekventering biblioteker for Nucleosome tæthed analyse

Article DOI: 10.3791/56085-v • 10:05 min • December 12th, 2017
December 12th, 2017 •
1, 1, 1, 1, 1,2
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency

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Vi præsenterer en modificeret indfødte kromatin immunoprecipitation sekventering (ChIP-seq) metode for generation af sekvens datasæt egnet til en nucleosome tæthed ChIP-seq analytiske rammer, som integrerer micrococcal nukleasen (MNase) tilgængelighed med Histon ændring målinger.

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Genetik spørgsmålet 130 Epigenomics kromatin immunoprecipitation Histon modifikation nucleosome tæthed cellulære heterogenitet micrococcal nukleasen
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