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In Vitro Characterization of the Electrophysiological Properties of Colonic Afferent Fibers in Rats

Article DOI: 10.3791/56090-v • 08:19 min • September 27th, 2017
September 27th, 2017 •
1, 1, 1, 1, 1, 1
1Hongqiao International Institute of Medical Research, Tongren Hospital and Department of Physiology, Faculty of Basic Medical Sciences, Shanghai Jiaotong University School of Medicine

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Summary
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Abnormal sensory function underlies visceral pain and other symptoms of functional and inflammatory bowel diseases. A protocol for the electrophysiological recording of the colonic afferent nerves in an ex vivo rat colorectum preparation is presented here.

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In Vitro Characterization Electrophysiological Properties Colonic Afferent Fibers Rats Sensory Nerves Colorectum Electrophysiological Recordings Methodology In Vitro Pen Field Outer Peripheral Efferens Sensitivity Correct Hyperalgesia Technique Distinction Carotid Distinction In Vivo Animals Patients Visual Demonstration Locating Ganglia Dissecting Nerves Abdominal Cavity Midline Incision Scalpel Mesentery Dissecting Microscope Left Pelvic Ganglion Pelvic Nerves Lumbar Splanchnic Nerves Symphysis Bone Rectum
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