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In Vivo Identifiering och analys av Rb proteinet SUMOylation i mänskliga celler
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In Vivo Identifiering och analys av Rb proteinet SUMOylation i mänskliga celler

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Liten ubiquitin-relaterade modifierare (SUMO) familj proteiner konjugeras till lysin resthalter av målproteiner att reglera olika cellulära processer. Detta dokument beskriver ett protokoll för detektion av retinoblastom (Rb) protein SUMOylation under endogena och exogena förhållanden i mänskliga celler.

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Molekylärbiologi frågan 129 retinoblastom protein (Rb) post-translationella modifieringen (PTM) små ubiquitin-relaterade modifierare (SUMO) cellcykeln HKE293 celler
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