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JoVE Journal Biology
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In Vivo Identifiering och analys av Rb proteinet SUMOylation i mänskliga celler

Article DOI: 10.3791/56096
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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November 2nd, 2017

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Liten ubiquitin-relaterade modifierare (SUMO) familj proteiner konjugeras till lysin resthalter av målproteiner att reglera olika cellulära processer. Detta dokument beskriver ett protokoll för detektion av retinoblastom (Rb) protein SUMOylation under endogena och exogena förhållanden i mänskliga celler.

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