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In Vivo Rilevamento e analisi di Rb sumoilazione di proteine in cellule umane
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In Vivo Rilevamento e analisi di Rb sumoilazione di proteine in cellule umane

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Proteine della famiglia piccolo ubiquitina-relativo modificatore (SUMO) sono coniugati ai residui di lisina delle proteine bersaglio per regolare i vari processi cellulari. Questo articolo descrive un protocollo per la rilevazione della proteina del retinoblastoma (Rb) sumoilazione in condizioni endogene ed esogene in cellule umane.

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Biologia molecolare problema 129 proteina del Retinoblastoma (Rb) modificazione post-traduzionale (PTM) piccolo ubiquitina-relativo modificatore (SUMO) ciclo cellulare le cellule HKE293
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