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I Vivo Deteksjon og analyse av Rb Protein SUMOylation i menneskeceller
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I Vivo Deteksjon og analyse av Rb Protein SUMOylation i menneskeceller

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Små ubiquitin-relaterte modifikator (SUMO) familie proteiner er konjugert til lysin rester av målet proteiner til å regulere ulike cellulære prosesser. Dette dokumentet beskriver en protokoll for påvisning av retinoblastoma (Rb) protein SUMOylation under endogene og ytre forhold i menneskeceller.

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Molekylærbiologi problemet 129 Retinoblastoma protein (Rb) post-translasjonell modifikasjon (PTM) liten ubiquitin-relaterte modifikator (SUMO) cellen syklus HKE293 celler
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