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JoVE Journal
Developmental Biology

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C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型
 
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C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型: 抗体染色、rna 干扰功能分析和成像标记

Article DOI: 10.3791/56100-v 12:15 min October 3rd, 2017
October 3rd, 2017

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Summary

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透明的C. 线虫小肠可作为 "体内组织室", 用于研究多细胞 tubulogenesis 中的单细胞和亚 apicobasal 膜和腔生物。本协议描述了如何结合标准标记, 功能的遗传/rna 干扰和微观的方法来解剖这些过程的分子水平。

Tags

发育生物学 问题 128 Tubulogenesis 上皮极性 膜生物学 发育遗传学 单细胞分析 原位成像
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