Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Counting Proteins in Single Cells with…
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.

JoVE Journal Cancer Research
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Inferior Vena Cava (IVC) Ligation for Venous Thrombosis Analysis in Mouse
Journal (Cancer Research)
Inferior Vena Cava (IVC) Ligation for Venous Thrombosis Analysis in Mouse
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Journal (Cancer Research)
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
 

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Article DOI: 10.3791/56110-v • 12:25 min • July 6th, 2018
July 6th, 2018 •
*1, *1, 1, 1
1Institute of Chemical Biology, Department of Chemistry, Imperial College London
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.

Tags

Proteins Single Cells Addressable Droplet Microarrays Protein Copy Number Single-cell Resolution Gene Expression Heterogeneity Single-cell Assays Antibody Microarrays Fused Silica Tubing Concentric Nozzle
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter