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JoVE Journal Neuroscience
Generazione e manutenzione a lungo termine di nervi liberi
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Generazione e manutenzione a lungo termine di nervi liberi

Article DOI: 10.3791/56115-v • 06:33 min • July 7th, 2017
July 7th, 2017 •
1, 1, 2, 1,2
1Division of Biological Sciences, UC San Diego, 2Physics Department, UC San Diego

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Attraverso un doppio trattamento con colchicina, può essere generata una tossina derivata da piante che uccide cellule divisorie, senza idrografia Hydra vulgaris . Questi Hydra non possono nutrire o esaminare da soli. Questo documento descrive un metodo migliorato per la manutenzione a lungo termine di Idra vulgaris privo di nervi in ​​laboratorio.

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Neuroscienze Numero 125, Colchicina alimentazione cellule interstiziali prive di nervi immunohistochemistry
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