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Journal / Immunology and Infection / In Situ MHC-tetrameer kleuring en kwantitatieve…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ MHC-tetrameer kleuring en kwantitatieve analyse om te bepalen van de locatie, overvloed en fenotype van CD8 Antigen-specifieke T-cellen in weefsels
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In Situ MHC-tetrameer kleuring en kwantitatieve analyse om te bepalen van de locatie, overvloed en fenotype van CD8 Antigen-specifieke T-cellen in weefsels

Article DOI: 10.3791/56130-v • 08:37 min • September 22nd, 2017
September 22nd, 2017 •
1, 1, 1
1Department of Veterinary and Biomedical Sciences, University of Minnesota

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Hier beschrijven we een methode die in situ MHC-tetrameer kleuring combineert met immunohistochemistry lokalisatie, fenotype, en hoeveelheid van antigeen-specifieke T-cellen in weefsels te bepalen. Dit protocol wordt gebruikt om te bepalen van de ruimtelijke en fenotypische kenmerken van antigeen-specifieke CD8 T-cellen ten opzichte van andere celtype en structuren in de weefsels.

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Immunologie kwestie 127 In situ tetrameer kleuring (IST) virus-specifieke CD8 + T cellen immunohistochemistry lokalisatie fenotype confocal microscopie kwantitatieve beeldanalyse
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