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El murino colina deficientes, suplementada con Ethionine (CDE) dieta de modelo de la lesión hepática crónica
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El murino colina deficientes, suplementada con Ethionine (CDE) dieta de modelo de la lesión hepática crónica

Article DOI: 10.3791/56138-v • 07:28 min • October 21st, 2017
October 21st, 2017 •
1, 1, 2, 3, 1,4, 1, 5,6,7, 8,9, 10,11, 1,12
1School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute, Curtin University, 3Institute of Biochemistry, Christian-Albrechts-University, 4School of Veterinary and Life Sciences, Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences, The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences, Edith Cowan University, 12School of Medicine and Pharmacology, University of Western Australia

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Aquí describimos un método común para inducir lesión hepática crónica en ratones por la alimentación de una dieta de deficiencia de colina y suplementada con ethionine (CDE). Demostramos la vigilancia de la salud, la perfusión hepática, aislamiento y conservación. Un curso de tiempo de seis semanas puede informar sobre la lesión del higado, pathohistology, fibrosis, inflamación y las respuestas de células progenitoras hepáticas.

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Biología celular número 128 modelo murino dieta suplementada con ethionine deficiencia de colina enfermedad hepática crónica la perfusión hepática aislamiento hígado células progenitoras hepáticas respuesta inflamatoria histología fibrosis microambiente hepático
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