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Real-time In Vivo optagelse af Arabidopsis calciumsignaler under insekt fodring ved hjælp af en fluorescerende Biosensor

Article DOI: 10.3791/56142-v • 08:21 min • August 15th, 2017
August 15th, 2017 •
1, 1, 2,3,4, 1, 5, 2, 1, 5, 1
1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

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Denne protokol beskriver en simpel metode til at analysere calciumsignaler i planter genereret ved at fodre hemipteran insekter såsom bladlus. Arabidopsis thaliana omdannet med normal god landbrugspraksis calcium biosensor GCaMP3 giver mulighed for real-time i vivo billeddannelse af calcium dynamics med en høj tidsmæssige og rumlige opløsning.

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Biokemi sag 126 Calcium bladlus GCaMP mikroskopi insekt normal god landbrugspraksis
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