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Journal / Biology / Realtid i Vivo inspelning av Arabidopsis kalcium…
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Realtid i Vivo inspelning av Arabidopsis kalcium signaler under insekt utfodring med hjälp av en fluorescerande Biosensor
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Realtid i Vivo inspelning av Arabidopsis kalcium signaler under insekt utfodring med hjälp av en fluorescerande Biosensor

Article DOI: 10.3791/56142-v • 08:21 min • August 15th, 2017
August 15th, 2017 •
1, 1, 2,3,4, 1, 5, 2, 1, 5, 1
1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

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Detta protokoll beskriver en enkel metod för att analysera kalcium signaler i växter som genereras av utfodring Ditropis insekter, såsom bladlöss. Arabidopsis thaliana omvandlas med den GFP kalcium biosensor GCaMP3 möjliggör av realtid i vivo imaging av kalcium dynamics med en hög temporal och spatial upplösning.

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Biokemi fråga 126 kalcium bladlöss GCaMP mikroskopi insekt GFP
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