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Journal / Bioengineering / Syntese af smitsomme Bakteriofager i en E. coli…
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Bioengineering

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JoVE Journal Bioengineering
Syntese af smitsomme Bakteriofager i en E. coli -baseret celle-gratis Expression System
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Syntese af smitsomme Bakteriofager i en E. coli -baseret celle-gratis Expression System

Article DOI: 10.3791/56144-v • 11:33 min • August 17th, 2017
August 17th, 2017 •
1, 2, 1, 2, 1
1School of Physics and Astronomy, University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of Minnesota

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En ny generation af platforme, celle-fri transskription-oversættelse er blevet manipuleret til at konstruere biokemiske systemer i vitro gennem udførelse af genet kredsløb. I denne artikel beskriver vi, hvordan Bakteriofager, MS2, ΦΧ174 og T7, er syntetiseret fra deres genom ved hjælp af en alle E. coli celle-fri TXTL system.

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Bioteknologi sag 126 celle-fri transskription-oversættelse (TXTL) bacteriophage E. coli plaque assay phage forstærkning phage genom rensning syntetisk biologi
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